What you'll learn
- Explain DNA structure and the biological rationale for sequencing
- Compare Sanger, short-read, and long-read sequencing technologies
- Outline every step in a basic next-generation sequencing (NGS) workflow
- Interpret FASTQ quality scores and common sequencing file formats
- Describe a core bioinformatics pipeline from raw reads to variant calls
- Discuss clinical, research, and industrial applications alongside ethical considerations
Curriculum
Module 1: Foundations of DNA and Sequencing3 topics
- DNA Structure & Genetic Information
- Why Sequence DNA? Real-World Drivers
- Milestones: From Sanger to the Genomic Era
Module 2: Sequencing Technologies & Wet-Lab Workflows3 topics
- Sanger Sequencing Basics
- Next-Generation Sequencing Fundamentals
- Long-Read & Emerging Technologies
Module 3: Data Formats & Core Bioinformatics Pipeline3 topics
- Sequencing Data Formats & Quality Scores
- Core Pipeline: QC → Alignment → BAM
- Variant Calling & Basic Interpretation
Module 4: Applications, Ethics, and the Future3 topics
- Clinical & Research Applications
- Data Governance & Ethical Considerations
- Future Trends & Career Pathways
About this course
A four-week, Socratic-style course that builds a solid, example-driven foundation in modern DNA sequencing—covering biology, technology, data, and real-world impact.
Prerequisites
- •Introductory biology (cell structure, central dogma)
- •High-school/first-year chemistry
- •Basic computer literacy (file navigation, spreadsheets)